Genomic DNA was extracted by using QIAamp DNA Minikit (Qiagen, Hilden, Germany), and sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs, NEB, USA) following the manufacturer’s recommendations. The whole genomes of S. pneumoniae were sequenced using the Illumina NovaSeq PE150 platform (Illumina, San Diego, CA, USA) with approximately 200× coverage at Beijing Novogene Bioinformatics Technology Co., Ltd.
Genome data were assembled and annotated as previously reported (Cui et al., 2022 (link)) with SPAdes software (v3.14.1) (Prjibelski et al., 2020 (link)) and Prokka software (v1.14.5) (Seemann, 2014 (link)). All of the assembled genomes were submitted to GenBank and approved (PRJNA914101, PRJNA915833 and PRJNA915821).3
Genome data were assembled and annotated as previously reported (Cui et al., 2022 (link)) with SPAdes software (v3.14.1) (Prjibelski et al., 2020 (link)) and Prokka software (v1.14.5) (Seemann, 2014 (link)). All of the assembled genomes were submitted to GenBank and approved (PRJNA914101, PRJNA915833 and PRJNA915821).
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