Imaging and 3D reconstructions of negatively stained GroEL, β-Galactosidase, and 20S Proteasome were performed as previously described.14 (link) Briefly, images were collected on a Technai G2 Spirit BioTwin (Thermo Fisher Scientific) defoci ranging from 0.5μm to 2μm using SerialEM software version 3.8.7.(Acquisition settings summarized in Supplementary Table 1 .) and 3D reconstruction was performed using cis-TEM (https://cistem.org/ ). Model fitting and figure creation was performed on UCSF ChimeraX version 1.3 (https://www.cgl.ucsf.edu/chimerax/ ).
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Structural Analysis of Macromolecular Complexes
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Corresponding Organization : Morgridge Institute for Research
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- Imaging and 3D reconstructions of negatively stained GroEL, β-Galactosidase, and 20S Proteasome
- Images collected on a Technai G2 Spirit BioTwin (Thermo Fisher Scientific) at defoci ranging from 0.5μm to 2μm
- 3D reconstruction performed using cis-TEM
- SerialEM software version 3.8.7 for image acquisition
- UCSF ChimeraX version 1.3 for model fitting and figure creation
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