Protein Extraction and Western Blot Analysis
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Corresponding Organization : Eli Lilly (United States)
Other organizations : Chinese Academy of Sciences, Shanghai Institutes for Biological Sciences, Discovery Institute, Sanford Burnham Prebys Medical Discovery Institute
Variable analysis
- Protein extractions
- Phospho-Akt (Ser473) levels
- Total Akt levels
- GALE levels
- Tubulin levels
- O-linked glycosylation levels
- Actin levels
- Primary antibodies used at 1:1,000 dilutions
- Secondary immunoglobulin G labeled with infrared dyes used at 1:10,000 dilutions
- Visualization using LI-COR Odyssey infrared scanner
- Data analysis using Odyssey version 3.0 software
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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