Extraction of Turnip Bioactive Compounds

The extraction method was adapted from [20 (link)]. Three replicates of each batch were prepared as follows: 40 mg of ground steamed-pureed turnip powder was heated in a dry-block at 75 °C for 2 min to ensure inactivation of any remaining active myrosinase enzyme. Preheated 70% (v/v) methanol (1.2 mL; 70 °C) was added and the sample placed in a water bath for 20 min at 70 °C. Samples were then centrifuged for 10 min (10,000 rpm, 18 °C) to collect loose material into a pellet. The supernatant was then filtered through 0.22 μm Acrodisc syringe filters with Supor membrane (hydrophilic polyethersulfone; VWR, Lutterworth, UK) and frozen (−80 °C) in Eppendorf tubes until analysis by LC-MS.