Oxygen Consumption Rate Measurement

The measurement of oxygen consumption rate was performed at 27°C by high-resolution respirometry (Oxygraph-2k series C and G, OROBOROS Instruments, Innsbruck, Austria). Cultures derived from mononucleate ascospores of wild type, ΔPaSnf1, ΔPaClpP, and ΔPaSnf1/ΔPaClpP were grown on M2 plates covered with a cellophane foil for 2 days, shifted into CM liquid medium, and further incubated for 3 days at 27°C under shaking and constant light. A small piece of mycelium was placed into the Oroboros respirometer chamber into 2 ml fresh CM media. The oxygen consumption rate was determined as previously described in Fischer et al. (2015a) (link). After the measurement, the mycelium was placed into a 2-ml tube and boiled for 10 min at 95°C. Subsequently, the mycelium was air-dried for 2 days, and dry weight was determined. Finally, basal oxygen consumption per mg mycelium was calculated. For the analysis of the data, the DatLab6 software from Oroboros (Innsbruck, Austria) was used.