Characterization of AML cell lines with IDH1/2 mutations

Human AML cell lines HL60, U937 were purchased at DSMZ (Braunschweig, Germany) and KG1a at ATCC (Manassas, VA, USA). THP1 expressing IDH1^WT were gifted by Prof. Steven Chan [11 (link)]. Clones from the HL60 cell line expressing either IDH1^WT (clone 2 and 7) or IDH1^R132H (clone 5 and 11) were generated by our team [4 (link)]. HL60 was engineered to express IDH2^WT or IDH2^R172K as described below. All the cells excluding THP1 were maintained in MEMα with Glutamax (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS (Invitrogen, Waltham, MA, USA) in the presence of 100 U/mL of penicillin and 100 µg/mL of streptomycin. THP1 were cultivated in RPMI with Glutamax (Gibco, Life Technologies, CA, USA) supplemented with 10% FBS (Invitrogen, MA, USA) in the presence of 100 U/mL of penicillin and 100 µg/mL of streptomycin. All cell lines have been routinely tested for mycoplasma contamination in the laboratory.