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UPLC-Based Polyphenol Analysis Protocol

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For the detection and quantification of the listed polyphenols, a Waters Acquity UPLC system (Waters Corp., Milford, MA, USA) equipped with an autosampler chamber, two pumps and a degasser, was used. The chromatographic separation was performed on an ACQUITY UPLC BEH C18 (100 x 2.1 mm, particle size: 1.7 μm) column (Waters Corp., Milford, MA, USA), heated at 30°C and eluted as it was previously reported with some modification for polyphenolic compounds, intact GLs and ITCs respectively (Song et al., 2005 (link); Tian et al., 2005 (link); Zhu et al., 2020 (link)).

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Kyriakou S., Michailidou K., Amery T., Stewart K., Winyard P.G., Trafalis D.T., Franco R., Pappa A, & Panayiotidis M.I. (2022). Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress). Frontiers in Plant Science, 13, 998755.

Publication 2022

Waters Acquity UPLC system ACQUITY UPLC BEH C18

Chromatographic Polyphenols

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Variable analysis

independent variables

Waters Acquity UPLC system (Waters Corp., Milford, MA, USA) equipped with an autosampler chamber, two pumps and a degasser
ACQUITY UPLC BEH C18 (100 x 2.1 mm, particle size: 1.7 μm) column (Waters Corp., Milford, MA, USA)
Column temperature at 30°C

dependent variables

Detection and quantification of the listed polyphenols

control variables

Chromatographic separation conditions as previously reported with some modification for polyphenolic compounds, intact GLs and ITCs

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