Six weeks old nude (Rj:NMRI-Foxn1 nu/nu) female mice were used for xenograft experiments. Five million Panc1 cells, in 25% of matrigel (Sigma Aldrich), were subcutaneously injected into the flank of mice. Treatment: gemcitabine at 100 mg/kg three times a week for Fig. 3 a-b and gemcitabine at 100 mg/kg or 50 mg/kg per intraperitoneal injection, once a week, and/or ABT-263 at 35 mg/kg or 50 mg/kg, per gavage, twice a week for Fig. 6 . Prior to treatments, randomized groups with an average tumor size of 80 mm3 were defined. Tumors were measured after 5 weeks of treatment. Tumor volume was calculated using the following formula: V = a*b*c/2, where “a” is the longest diameter, “b” is the shortest one, and c the depth. Relative tumor volumes (vs tumor volume at the beginning of the treatments) were then calculated. Mice were maintained in laminar-flow boxes under standard conditions (standard diet and water ad libitum) in our specific pathogen-free animal house. Experiments were performed according to animal care guidelines of European and French laws. Protocols were authorized by the local animal ethic evaluation committee (CECCAPP:CLB_2017_041) and by the French ministry of education and research (APAFIS#12774).
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