The immunohistochemistry was carried out using mouse monoclonal anti-CXCR4 (1:100 dilution; Abcam, Cambridge, UK) and rabbit polyclonal anti-CXCR7 (1:200 dilution; GeneTex, Irvine, CA, USA) as primary antibodies. Isotype control was performed using mouse IgG1k (MOPC-21; 1:50 dilution; Abcam, Cambridge, UK) and rabbit immunoglobulin fraction (Code X0903; 1:1000 dilution; Dako, Glostrup, Denmark). CXCR4 expressing tonsil tissue and CXCR7 expressing pancreatic adenocarcinoma served as positive controls. The prognostic power of CXCR4 and CXCR7 was assessed in accordance with the REporting recommendations for tumour MARKer prognostic studies (REMARK)24 .
Immunohistochemical Analysis of CXCR4 and CXCR7 in Follicular Thyroid Carcinoma
The immunohistochemistry was carried out using mouse monoclonal anti-CXCR4 (1:100 dilution; Abcam, Cambridge, UK) and rabbit polyclonal anti-CXCR7 (1:200 dilution; GeneTex, Irvine, CA, USA) as primary antibodies. Isotype control was performed using mouse IgG1k (MOPC-21; 1:50 dilution; Abcam, Cambridge, UK) and rabbit immunoglobulin fraction (Code X0903; 1:1000 dilution; Dako, Glostrup, Denmark). CXCR4 expressing tonsil tissue and CXCR7 expressing pancreatic adenocarcinoma served as positive controls. The prognostic power of CXCR4 and CXCR7 was assessed in accordance with the REporting recommendations for tumour MARKer prognostic studies (REMARK)24 .
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Corresponding Organization : Düsseldorf University Hospital
Variable analysis
- None explicitly mentioned
- Expression levels of CXCR4 and CXCR7
- Formalin-fixed paraffin-embedded tissue samples obtained from the Institute of Pathology, University Hospital Duesseldorf
- Diagnosis of FTC histologically confirmed
- Construction of the tissue microarray, immunohistochemistry and protein expression analyses performed as described previously
- CXCR4 expressing tonsil tissue
- CXCR7 expressing pancreatic adenocarcinoma
- Mouse IgG1k (MOPC-21) isotype control
- Rabbit immunoglobulin fraction (Code X0903) isotype control
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