Western Blot Analysis of Protein Expression

Western blot analysis was performed as described [7 (link)]. Proteins were detected by the Odyssey 9120 Infrared Imaging System (Li-Cor Biosciences, Lincoln, Nebraska, USA). The antibodies used were anti-MGMT (Merck Millipore, Billerica, Massachusetts, USA), anti-HSP90, anti-beta-actin (Santa Cruz Biotechnology, Heidelberg, Germany), anti-MSH2 (Calbiochem, San Diego, CA, USA), anti-MSH6 (Transduction Laboratories, Lexington, KY, USA) and anti-MLH1 (BD Pharmingen, Heidelberg, Germany).

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Roos W.P., Quiros S., Krumm A., Merz S., Switzeny O.J., Christmann M., Loquai C, & Kaina B. (2014). B-Raf inhibitor vemurafenib in combination with temozolomide and fotemustine in the killing response of malignant melanoma cells. Oncotarget, 5(24), 12607-12620.

Publication 2014

Odyssey 9120 Infrared Imaging System anti-HSP90 anti-beta-actin anti-MLH1

Antibodies Beta actin Hsp90 Mgmt Mlh1 Msh6 Proteins Western blot analysis

Corresponding Organization :

Other organizations : Johannes Gutenberg University Mainz, University Medical Center of the Johannes Gutenberg University Mainz

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Variable analysis

independent variables

Antibody types used for protein detection

dependent variables

Protein expression levels detected by the Odyssey 9120 Infrared Imaging System

control variables

Western blot analysis protocol as described in the referenced study [7]

controls

No positive or negative controls were explicitly mentioned in the information provided.

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