Microarray Analysis of RCC Cells

CD105+ cells were isolated from ACHN (metastatic RCC) and Caki-2 (primary RCC). A healthy kidney epithelial cell line (ASE) was used as a control for both RCC cell lines. RNA was extracted using Total RNA Mini Plus (A&A Biotechnology, Gdynia, Poland). RNA quality and integrity were measured by BioAnalyzer 2100 (Agilent, California, USA) before a microarray experiment was carried out. Amplification, labeling, generation of cRNA, and hybridization were done by PERLAN Technologies (Warsaw, Poland) on Agilent’s human GE 4x44K v2 (G4845A) (California, USA) microarrays, as described previously by Stankiewicz et al. [34 (link)].