Anti-ATL2 rabbit polyclonal antibodies were custom-raised against the his6-tagged cytosolic domain (1−479 a.a.) of ATL2 (AbFrontier). These antibodies were affinity-purified using their antigens bound to SulfoLink resin (20401; Thermo Fisher Scientific). Anti-ATL3 rabbit polyclonal (ab117819; Abcam), anti-myc mouse monoclonal (2276S; Cell Signaling technology), anti-GFP mouse monoclonal (MA5-15256; Invitrogen), anti-Gluc rabbit polyclonal (E8023; New England BioLabs), anti-calnexin mouse monoclonal (sc-23954; Santa Cruz), anti-GAPDH mouse monoclonal (MA5-15738; Thermo Fisher Scientific), and anti-protein disulfide isomerase mouse monoclonal (MA3-019; Invitrogen) antibodies were purchased. Anti-Yet3p rabbit sera were a generous gift from C. Barlowe (Geisel School of Medicine at Dartmouth). Alexa Fluor 594-conjugated anti-mouse goat IgG (A-11005) was purchased from Invitrogen. Peroxidase-conjugated anti-mouse goat IgG (AB_10015289) and peroxidase-conjugated anti-rabbit goat IgG (AB_2313567) were purchased from Jackson ImmunoResearch.
Protocol detail
ATL2 Antibody Production and Characterization
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Alexa 594
Anti antibodies
Anti igg
Antibodies
Antigens
Calnexin
Cytosolic
Gapdh
Goat
Mouse
Peroxidase
Protein disulfide isomerase
Rabbit
Resin
Sera
Corresponding Organization : Gwangju Institute of Science and Technology
Other organizations : Ulsan National Institute of Science and Technology
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Variable analysis
independent variables
- Anti-ATL2 rabbit polyclonal antibodies raised against the his6-tagged cytosolic domain (1−479 a.a.) of ATL2
- Anti-ATL3 rabbit polyclonal antibody (ab117819; Abcam)
- Anti-myc mouse monoclonal antibody (2276S; Cell Signaling technology)
- Anti-GFP mouse monoclonal antibody (MA5-15256; Invitrogen)
- Anti-Gluc rabbit polyclonal antibody (E8023; New England BioLabs)
- Anti-calnexin mouse monoclonal antibody (sc-23954; Santa Cruz)
- Anti-GAPDH mouse monoclonal antibody (MA5-15738; Thermo Fisher Scientific)
- Anti-protein disulfide isomerase mouse monoclonal antibody (MA3-019; Invitrogen)
- Anti-Yet3p rabbit sera
- Not explicitly mentioned
- Affinity-purified anti-ATL2 rabbit polyclonal antibodies using their antigens bound to SulfoLink resin (20401; Thermo Fisher Scientific)
- Alexa Fluor 594-conjugated anti-mouse goat IgG (A-11005; Invitrogen)
- Peroxidase-conjugated anti-mouse goat IgG (AB_10015289; Jackson ImmunoResearch)
- Peroxidase-conjugated anti-rabbit goat IgG (AB_2313567; Jackson ImmunoResearch)
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