Generating Proximal Tubule-Specific AT1a Receptor-Deficient Mice

Wild-type (C57BL/6J or littermates) and global AT_1a receptor-deficient mice, Agtr1a^−/− or B6.129P2-Agtr1a^tm1Unc/J (Stock No: 002682), were purchased from Jackson Laboratories and bred in this laboratory as previously described.^{27 (link),28 (link)} Proximal tubule-specific AT_1a receptor-deficient mice, PT-Agtr1a^−/−, were generated and bred in the this laboratory using the iL1-Sglt2-Cre/Agtr1a-floxed approach, as we described recently for PT-Nhe3^−/− mice.^{13 (link),14 (link)} The iL1-Sglt2-Cre mice were originally obtained from Rubera et al., ^{25 (link)} whereas Agtr1a-floxed mice, C57BL/6N-Agtr1a^tm1Uky/J (Stock No: 016211), were generated by Rateri et al. ^{29 (link)} and purchased from Jackson laboratories. C57BL/6N-Agtr1a^tm1Uky/J mice were crossed with iL1-Sglt2-Cre mice to generate PT-Agtr1a^−/− mice for the studies in the present study (Figures S1–S3). The use of adult male (>12-week-old) wild-type, global Agtr1a^−/−, and PT-Agtr1a^−/− mice were approved by the Institutional Animal Care and Use Committees of the University of Mississippi Medical Center and Tulane University School of Medicine, respectively.