Western Blot Analysis of Testicular Proteins

Testicular lysates and immunoprecipitated proteins were separated by SDS-PAGE and transferred onto a polyvinylidene difluoride membrane as previously reported (31 (link),32 (link)). The membrane was blocked and incubated overnight at 4 °C with the indicated primary antibodies (Table S1). The membrane was washed and incubated at room temperature for 2 h with secondary antibodies. The signals of the detected proteins were visualized on the SuperSignal West Femto Chemiluminescent Substrate detection system (Thermo Scientific).

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Shen C., Xu J., Zhou Q., Lin M., Lv J., Zhang X., Wu Y., Chen X., Yu J., Huang X, & Zheng B. (2021). E3 ubiquitin ligase ASB17 is required for spermiation in mice. Translational Andrology and Urology, 10(12), 4320-4332.

Publication 2021

SuperSignal West Femto Chemiluminescent Substrate detection system

Antibodies Lysates Membrane Polyvinylidene difluoride Proteins Sds page

Corresponding Organization : Nantong University

Other organizations : Nanjing Maternity and Child Health Care Hospital, Soochow University, Affiliated Hospital of Nantong University

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