Diabetic Wound Healing Model Development

Diabetic rat models were established according to the classic modeling method [14 (link)]. After 3 days of acclimatization, the 8‑week‑old mice were fed a high-fat and high-sugar diet consisting of 54.6% basic mouse feed, 16.9% lard, 14% sugar, 10.2% casein, 2.1% premix, and 2.2% maltodextrin for two weeks. Then, the diabetic model was induced by intraperitoneal injection of 1% STZ solution of 50 mg/kg and intragastric administration of 10% glucose solution 2 h later to balance blood glucose. The weight of the rats was measured on the day of modeling, and tail tip blood was collected to measure blood glucose.
One week after the last STZ injection, rats with blood glucose levels over 16.7 mmol/L, polyuria, polydipsia and intense hunger symptoms were considered to have successfully developed diabetes mellitus. On the day of modeling, rats were weighed and anesthetized with 2%-3% isoflurane. After anesthesia, the model area (both sides of the spine) was depilated with a depilatory knife. Under aseptic conditions, skin wounds with a diameter of 0.6 cm were made into diabetic rats with an area of 0.28 cm². Each rat had 6 holes on the back. The depth of the wound reached the level of the subfascial dressing, and every rat was fed continuously in an individual cage separately. Intergroup markers should be made for high-fat and high-sugar diets.