The SARS-CoV-2 PLpro FRET substrate Dabcyl-FTLRGG/APTKV(Edans) and the SARS-CoV-2 Mpro FRET substrate Dabcyl-KTSAVLQ/SGFRKME(Edans) were synthesized by solid-phase synthesis through iterative cycles of coupling and deprotection using the previously optimized procedure.27 (link) Ub-AMC and ISG15-AMC were purchased from Boston Biochem (catalog # U-550-050 and UL-553-050 respectively).
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