The specific primary antibodies against E-cadherin (ET1607–75), N-cadherin (ET1607–37), snail (ER1706–22), Flag (M1403–2), p-AKT(ET1607–73), AKT (ET1609–51), p-ERK (ET1610–13), ERK (ET1601–29), p-MEK (ET1609–50), CXCR4 (ER1802–28) and ENO1 (ET1705–56) were ordered from Hangzhou Hua-An Biotechnology Company. Other antibodies included β-actin (TA-09, ZSGB-BIO), MEK (sc-436, Santa Cruz), HK2 (22029–1-AP, Proteintech), ALDOA (11217–1-AP, Proteintech) and PGK1 antibody (sc-130335, Santa Cruz).
For serum PGK1 detection, the high abundance of serum albumin and IgG was deleted [21 (link), 22 (link)] using a reagent kit following procedures (Albumin and IgG Erasin Kit, C500063–0005, Shanghai Sangon Biotechnology). Reversible Ponceau staining was used as a loading control.
The density of target band in Western blot was measured with Image J software (NIH, USA) for semi-quantification of staining signals.
For serum PGK1 detection, the high abundance of serum albumin and IgG was deleted [21 (link), 22 (link)] using a reagent kit following procedures (Albumin and IgG Erasin Kit, C500063–0005, Shanghai Sangon Biotechnology). Reversible Ponceau staining was used as a loading control.
The density of target band in Western blot was measured with Image J software (NIH, USA) for semi-quantification of staining signals.
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