Jatropha Endosperm Total RNA Isolation

Total RNA was isolated from jatropha endosperm using methods described previously [32 (link)]. About 10 μg total RNA was fractionated on a 1.2 % formaldehyde agarose gel and blotted onto a Hybond^TM N⁺ membrane (Amersham Biosciences, Little Chalfont, Buchinghamshire, UK). The DNA probe for the C1 gene (C1 probe) for northern blot analysis was the PCR products amplified from jatropha genome with primers C1SP-F and C1SP-R (Table 1). The northern blot hybridization and the labelling of the C1 probe were similar to the methods described for the Southern blot analysis.