Multiparameter Immune Cell Analysis

PBMC staining and analysis were as described [3 (link)]. Unless otherwise specified, mAbs were from BD Pharmingen and included anti-CD3-Alexa Fluor 700 (UC-HT1), anti-CD4-APC (RPA-T4), anti-CD8-PacificBlue (RPA-T8), anti-CD11c-FITC (BU15, Thermo Scientific), anti-CD14-APC (RMO52, Beckman Coulter), anti-CD16-PacificBlue (3G8), anti-CD19-APC (HIB19) and anti-CD20-FITC (L27), anti-CD56-APC (N901, Beckman Coulter), and anti-NKG2D-PE (1D11; [2 (link)]). Anti-NKG2DL mAb were anti-MICA/B-PE (6D4), anti-ULBP1-PE (170818, R & D Systems), anti-ULBP2/5/6-PE (165903; R & D Systems), anti-ULBP3-PE (166510; R & D Systems), and anti-ULBP4-PE (1H1; [12 (link)]). In some cases, viable cell numbers were limiting resulting in partial data sets.

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Hamada S., Caballero-Benitez A., Duran K.L., Stevens A.M., Spies T, & Groh V. (2017). Soluble MICB in Plasma and Urine Explains Population Expansions of NKG2D+CD4 T Cells Inpatients with Juvenile-Onset Systemic Lupus Erythematosus. Open journal of immunology, 7(1), 1-17.

Publication 2017

anti-CD3-Alexa Fluor 700 (UC-HT1), anti-CD4-APC anti-CD8-PacificBlue anti-CD11c-FITC (BU15, anti-CD14-APC (RMO52, anti-CD16-PacificBlue (3 anti-CD19-APC anti-CD20-FITC anti-CD56-APC anti-MICA/B-PE (6D4 anti-ULBP1-PE anti-ULBP2/5/6-PE anti-ULBP3-PE

Anti cd3 Fitc Mica

Corresponding Organization :

Other organizations : University of the Ryukyus, Okinawa Prefecture, Fred Hutch Cancer Center, University of Washington, Seattle Children's Hospital

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Variable analysis

independent variables

Monoclonal antibodies (mAbs) used for staining: anti-CD3-Alexa Fluor 700, anti-CD4-APC, anti-CD8-PacificBlue, anti-CD11c-FITC, anti-CD14-APC, anti-CD16-PacificBlue, anti-CD19-APC, anti-CD20-FITC, anti-CD56-APC, anti-NKG2D-PE, anti-MICA/B-PE, anti-ULBP1-PE, anti-ULBP2/5/6-PE, anti-ULBP3-PE, and anti-ULBP4-PE

dependent variables

Expression levels of cell surface markers on PBMC samples

control variables

PBMC staining and analysis procedure as described in reference [3]
Monoclonal antibodies (mAbs) from BD Pharmingen, unless otherwise specified

positive controls

None specified

negative controls

None specified

Annotations

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