Annexin V-FITC Assay in DAOY Cells

DAOY cells were seeded onto eight wells coverslips for 24 h in media. Rhodamine-GuaDex was prepared as previously [21 (link)]. The cells were PBS washed, incubated with Rhodamine-GuaDex at 5 µM for 1 h in PBS, and then washed twice with PBS. Cells were then incubated with 10 µL Annexin V-FITC detection kit (Sigma-Aldrich, St. Louis MO, USA) in PBS for 30 min at 4 °C dark and RT. After staining, the cells were washed carefully and prepared with DAPI mounting medium. A confocal microscope (Leica SP5) was used for the image analysis.

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Gallo-Oller G., de Ståhl T.D., Alaiya A., Nilsson S., Holmberg A.R, & Márquez-Méndez M. (2023). Cytotoxicity of poly-guanidine in medulloblastoma cell lines. Investigational New Drugs, 41(5), 688-698.

Publication 2023

Annexin V-FITC detection kit

Annexin v fitc Cells Confocal microscope Dapi Rhodamine

Corresponding Organization :

Other organizations : Karolinska Institutet, King Faisal Specialist Hospital & Research Centre, Universidad Autónoma de Nuevo León

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Variable analysis

independent variables

Rhodamine-GuaDex concentration (5 µM)

dependent variables

Apoptosis (Annexin V-FITC staining)

control variables

Incubation time with Rhodamine-GuaDex (1 h)
Incubation time with Annexin V-FITC (30 min)
Incubation temperature with Annexin V-FITC (4 °C)
Cell type (DAOY cells)
Cell seeding time (24 h)
Cell culture medium
PBS washing

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