Protocol detail

Transcriptomic Analysis of Tibialis Anterior

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The tibialis anterior muscle sample at the different time points was homogenized, and RNA was extracted with RNasey Mini Kit (Qiagen, San Francisco, CA, United States) according to the manufacturer’s instructions. Microarray analysis was performed using an Agilent SurePrint G3 Rat GE (8 × 60K, Design ID: 028279), as previously described (Shen et al., 2019 (link)). Microarray analysis was performed on an Agilent Gene Chip platform and scanned by Agilent Scanner G2505C (Agilent Technologies). Data were extracted from scanned images using Agilent Feature Extraction Software (version 10.7.1.1, Agilent Technologies). The raw data were normalized by Genespring Software (version 13.1, Agilent Technologies). This dataset is available at the NCBI Gene Expression Omnibus (GEO) repository with GEO accession: GSE201025.

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Yang X., Li M., Ji Y., Lin Y., Xu L., Gu X., Sun H., Wang W., Shen Y., Liu H, & Zhu J. (2022). Changes of Gene Expression Patterns of Muscle Pathophysiology-Related Transcription Factors During Denervated Muscle Atrophy. Frontiers in Physiology, 13, 923190.

Publication 2022

RNasey Mini Kit SurePrint G3 Rat GE Agilent Scanner G2505C Agilent Feature Extraction Software Genespring Software

Gene chip Gene expression Microarray analysis Tibialis anterior muscle

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

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control variables

The tibialis anterior muscle sample was homogenized, and RNA was extracted with RNasey Mini Kit (Qiagen, San Francisco, CA, United States) according to the manufacturer's instructions.
Microarray analysis was performed using an Agilent SurePrint G3 Rat GE (8 × 60K, Design ID: 028279), as previously described (Shen et al., 2019).
Microarray analysis was performed on an Agilent Gene Chip platform and scanned by Agilent Scanner G2505C (Agilent Technologies).
Data were extracted from scanned images using Agilent Feature Extraction Software (version 10.7.1.1, Agilent Technologies).
The raw data were normalized by Genespring Software (version 13.1, Agilent Technologies).

controls

No positive or negative controls were explicitly mentioned in the provided information.

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