Quantifying Liver Protein Expression

Liver cytoplasmic extracts were prepared as described previously (Jouffe et al. 2013 (link)). Protein extract concentrations were quantified using a BCA protein assay kit (Thermo Fisher Scientific), and 20 µg of liver protein extract was resolved by SDS-PAGE using standard procedures. Densitometry analyses of the blots were performed using the ImageJ software. Naphtol blue and black staining of the membranes was used as a loading control and served as a reference for normalization of the quantified values. CRY1 antibody (1/500) was from Abcam (ab104736).

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Mermet J., Yeung J., Hurni C., Mauvoisin D., Gustafson K., Jouffe C., Nicolas D., Emmenegger Y., Gobet C., Franken P., Gachon F, & Naef F. (2018). Clock-dependent chromatin topology modulates circadian transcription and behavior. Genes & Development, 32(5-6), 347-358.

Publication 2018

BCA protein assay kit ab104736

Antibody Assay Cytoplasmic Densitometry Liver extract Membranes Naphtol Protein Sds page

Corresponding Organization :

Other organizations : École Polytechnique Fédérale de Lausanne, University of Lausanne

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Variable analysis

independent variables

Protein extract concentrations

dependent variables

CRY1 protein levels

control variables

Protein extract loading control (Naphtol blue and black staining)

controls

Positive control: Not specified
Negative control: Not specified

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