Immunostaining of Hematopoietic Cells

The presence of infiltrating human hemopoietic CD45⁺ cells (monoclonal rabbit anti-human CD45 antibody, 1:500, #ab40763; Abcam, Cambridge, UK) and T cells (XP^® monoclonal rabbit anti-human CD3ε (D7A6E™), 1:300, #85061; Cell Signalling Technology^® Danvers, MA, USA) in murine tissue was detected by immunohistochemistry (secondary Goat-anti-Rabbit IgG H&L (HPR) antibody, 1:1000, #ab 205718; Abcam, Cambridge, UK) and immunofluorescence (secondary Goat-anti-Rabbit IgG H&L (Alexa Fluor^®) antibody, 1:6500, #111-585-003; Jackson Immunoresearch, West Grove, PA, USA) in Formalin-Fixed Paraffin-Embedded tissue blocks as previously described [16 (link),17 (link)].

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Zeng B., Moi D., Tolley L., Molotkov N., Frazer I.H., Perry C., Dolcetti R., Mazzieri R, & Cruz J.L. (2023). Skin-Grafting and Dendritic Cell “Boosted” Humanized Mouse Models Allow the Pre-Clinical Evaluation of Therapeutic Cancer Vaccines. Cells, 12(16), 2094.

Publication 2023

ab40763 ab 205718

Anti igg Antibody Cd3ε Formalin Goat Hemopoietic Human Immunofluorescence Immunohistochemistry Monoclonal antibody Murine Paraffin Rabbit T cells Tissue

Corresponding Organization : University of Melbourne

Other organizations : Princess Alexandra Hospital

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Variable analysis

independent variables

Presence of infiltrating human hemopoietic CD45+ cells
Presence of T cells (CD3ε)

dependent variables

Detection of human hemopoietic CD45+ cells and T cells (CD3ε) in murine tissue

control variables

Formalin-Fixed Paraffin-Embedded tissue blocks

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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