Propagation of Influenza Viruses in MDCK Cells

Madin Darby
Canine Kidney (MDCK) cells obtained from the Center of Scientific
Excellence for Influenza Viruses, National Research Centre, Egypt
were grown in Dulbecco’s Modified Eagle’s Medium (DMEM)
(DMEM; BioWhittaker, Walkersville, MD, USA) supplemented with 10%
fetal bovine serum (FBS) (Gibco-BRL; New York, USA) and 2% penicillin/streptomycin
(pen/strep) antibiotic/antimycotic mixture (GIBCO-BRL; New York, USA)
at 37 °C/5% CO₂ in a humified incubator.
Seasonal
influenza A/Egypt/NRC098/2019(H1N1) (GISAID ID: EPI_ISL_12995118)
and highly pathogenic avian influenza A/chicken/Egypt/N12640A/2016(H5N1)
were obtained from the Center of Scientific Excellence for Influenza
Viruses, National Research Centre, Egypt, propagated in MDCK and embryonated
eggs, respectively, as previously described.^{7 (link)}