INI-4001 was synthesized and processed to over 99% purity as described previously [18 (link),29 (link)]. Hemagglutinin antigen trimer (H7) was obtained from Dr. Florian Krammer at the Icahn School of Medicine at Mount Sinai [30 (link),31 (link)]. All chemicals used were of analytical reagent grade and all solvents were of HPLC grade. Trifluoroacetic acid (TFA) and ammonium hydroxide were obtained from J.T. Baker (Phillipsburg, NJ, USA). Sterile water for irrigation (WFI) was obtained from Baxter Healthcare Corp (Deerfield, IL, USA). Methanol, anhydrous ethanol, isopropyl alcohol, Triton X-100, glycerol, ammonium formate, acetone, tetrahydrofuran, methyl t-butyl ether, and sodium hydroxide were obtained from Thermo Fisher Scientific (Waltham, MA, USA). (3-Aminopropyl) triethoxysilane (APTES) was obtained from Sigma-Aldrich (St. Louis, MO, USA). 50 and 200 nm solid SNP were purchased as ethanol suspensions (10 mg/mL) from NanoComposix (San Diego, CA, USA). RPMI medium 1640 (Cat. No. 11875-093, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) was supplemented with 10% fetal bovine serum (Cat. No. 35-011-CV, Corning Inc., Corning, NY, USA), 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL glutamine (Cat. No. 10378-016, Thermo Fisher) to prepare the complete RPMI media (RP-10).
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