Transcriptome Analysis of Valvular Heart Disease

To describe the transcriptome profile of patients with VHD, microRNAs will be extracted from platelet-free plasma and frozen valve fragments [59 (link), 60 (link)]. The miRNeasy Serum/Plasma and miRNeasy Tissue/Cells kits will be used for miRNA extraction according to the manufacturers’ protocols (Qiagen, Hilden, Germany). Targeted and untargeted transcriptome analysis will be used to quantify dysregulated miRNAs [59 (link)–62 (link)]. High-throughput techniques will be used to sequence the extracted miRNAs. Integrative bioinformatics will be used to determine the mRNAs targeted by the altered miRNAs in RHD and AS and correlate them to proteomics and metabolomics biomarkers [59 (link)–62 (link)]. Transcriptomic biomarkers with a significant q value (q <0.05 will be considered important.

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Mutithu D.W., Aremu O.O., Mokaila D., Bana T., Familusi M., Taylor L., Martin L.J., Heathfield L.J., Kirwan J.A., Wiesner L., Adeola H.A., Lumngwena E.N., Manganyi R., Skatulla S., Naidoo R, & Ntusi N.A. (2024). A study protocol to characterise pathophysiological and molecular markers of rheumatic heart disease and degenerative aortic stenosis using multiparametric cardiovascular imaging and multiomics techniques. PLOS ONE, 19(5), e0303496.

Publication 2024

miRNeasy Serum/Plasma

Corresponding Organization : Wellcome Centre for Infectious Diseases Research in Africa

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Variable analysis

independent variables

MicroRNAs extracted from platelet-free plasma and frozen valve fragments

dependent variables

Dysregulated miRNAs
MRNAs targeted by the altered miRNAs in RHD and AS
Proteomics and metabolomics biomarkers

control variables

MiRNA extraction protocols using the miRNeasy Serum/Plasma and miRNeasy Tissue/Cells kits according to the manufacturers' protocols (Qiagen, Hilden, Germany)

controls

No specific positive or negative controls were mentioned in the input.

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