Antibiotic Susceptibility of STEC Isolates

All PCR-confirmed STEC isolates were tested for antibiotic sensitivity. The susceptibility test was employed according to the Kirby-Bauer disc diffusion method using eight (amoxicillin (20 μg), ciprofloxacin (5 μg), streptomycin (10 μg), gentamicin (10 μg), ampicillin (25 μg), chloramphenicol (30 μg) (HiMedia, India), kanamycin (10 μg), and tetracycline (10 μg) (Oxoid, England)), commercially available antibiotic-impregnated disks. The antibiotics were selected based on the treatment of choice for E. coli strains. Antibiotics were also selected based on their availability and frequent utilization for treating disease in the area. Mueller Hinton (MHA) agar media (Oxoid, England) and a 0.5 Mac-Farland standard suspension of the bacteria were prepared to culture and adjust the density of the bacterial suspension, respectively. The result of the zone of inhibition was measured and interpreted as sensitive, intermediate, or resistant according to the Clinical and Laboratory Standards Institute (CLSI) guidelines [24 (link)].

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Yihunie F.B., Belete M.A., Fentahun G, & Dubie T. (2024). Molecular detection and antibiogram of Shiga toxin-producing Escherichia coli (STEC) from raw milk in and around Bahir Dar town dairy farms, Ethiopia. Heliyon, 10(7), e28839.

Publication 2024

amoxicillin ciprofloxacin streptomycin gentamicin ampicillin chloramphenicol kanamycin tetracycline

Corresponding Organization : Samara University

Other organizations : Debre Markos University

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Variable analysis

independent variables

Antibiotic-impregnated disks (amoxicillin (20 μg), ciprofloxacin (5 μg), streptomycin (10 μg), gentamicin (10 μg), ampicillin (25 μg), chloramphenicol (30 μg), kanamycin (10 μg), and tetracycline (10 μg))

dependent variables

Antibiotic susceptibility (measured as zone of inhibition)

control variables

Mueller Hinton (MHA) agar media
0.5 Mac-Farland standard suspension of the bacteria

controls

No positive or negative controls were explicitly mentioned in the information provided.

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