Western Blot Analysis of EMT Markers

Western blot analysis (WB) was carried out essentially as previously described in (63 (link)). The detection and quantification were performed with Odyssey Clx System (LI-COR Biosciences) through the Image Studio Software, or by traditional ECL detection. The following antibodies were used: anti-ZEB1-1642 (from Dr. Douglas Darling Lab), anti-ZEB1 (Santa Cruz,# sc-25388), anti-E-cadherin (BD Biosciences,# 610182), anti-vimentin (Cell Signaling, #5741), anti-SNAIL (Cell Signaling, #3879), anti β-catenin (Cell Signaling, #8480), anti ZO-1 (Cell Signaling, #8193), anti-cytokeratin 18 (Cell Signaling, #4548), anti-phospho serine/threonine (Abcam, #ab9337), anti-phospho-substrates antibodies kit Cell Signaling, #9920) anti-GFP (Abcam, #ab290), anti-PKCα (Santa Cruz, # sc-208), anti-PKCδ (Cell Signaling, #2058) anti-PKCε (Santa Cruz,# sc-1681), anti- Phospho-PKCα/β II (Cell Signaling, #9375) anti-α-tubulin (Sigma-Aldrich, #T5168); anti-β-actin (Sigma-Aldrich, #A2228); anti-PCNA (Santa Cruz, #sc56). As secondary antibodies we used anti-rabbit-Alexa-Fluor 594 (Molecular Probes), anti-rabbit HRP (Cell Signaling, #7074), anti-mouse HRP (Cell Signaling, #7076), goat anti-mouse IRDye 680RD and goat anti-rabbit IRDye 800CW (LI-COR Biosciences).