4TM Administration in Animal Study

The 4TM treatment (H6278, Sigma-Aldrich) was dissolved as previously described (29 (link), 68 (link)). The final solution contained 4TM (2.5 mg/ml), 5% dimethyl sulfoxide, and 1% Tween 80 in saline. Animals received 4TM (25 mg/kg, intraperitoneally) 2 hours after the punishment session.

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Domi E., Xu L., Toivainen S., Nordeman A., Gobbo F., Venniro M., Shaham Y., Messing R.O., Visser E., van den Oever M.C., Holm L., Barbier E., Augier E, & Heilig M. (2021). A neural substrate of compulsive alcohol use. Science Advances, 7(34), eabg9045.

Publication 2021

H6278

Animals Dimethyl sulfoxide Saline Tween 80

Corresponding Organization : University of Electronic Science and Technology of China

Other organizations : University of Edinburgh, University of Maryland, Baltimore, National Institute on Drug Abuse, The University of Texas at Austin, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

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Variable analysis

independent variables

4TM (2.5 mg/ml) concentration in the final solution

dependent variables

Not explicitly mentioned

control variables

5% dimethyl sulfoxide in the final solution
1% Tween 80 in the final solution
Saline as the vehicle for the final solution

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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