To analyze the populations of inflammatory cells within the placental tissue and spleen of pregnant mice, the mice were sacrificed under general anesthesia. Placental and splenic cells were
isolated from mice, as described previously [30 (link), 31 (link)]. The cells were washed, treated with erythrocyte lysis
buffer, and resuspended in PBS supplemented with 3% fetal bovine serum for flow cytometry analysis. Cells were labeled with the following antibodies purchased from eBioscience (San Diego,
CA, USA): phycoerythrin (PE)-conjugated anti-Ly6G (Cat 12-9668-82), PE-conjugated anti-CD11b (Cat 12-0112-82), FITC-conjugated anti-F4/80 (Cat 11-4801-82), and allophycocyanin-conjugated
anti-CD45 (Cat 17-0451-82). The cells were examined by flow cytometry using a NovoCyte flow cytometer (ACEA Biosciences Inc., San Diego, CA, USA).
isolated from mice, as described previously [30 (link), 31 (link)]. The cells were washed, treated with erythrocyte lysis
buffer, and resuspended in PBS supplemented with 3% fetal bovine serum for flow cytometry analysis. Cells were labeled with the following antibodies purchased from eBioscience (San Diego,
CA, USA): phycoerythrin (PE)-conjugated anti-Ly6G (Cat 12-9668-82), PE-conjugated anti-CD11b (Cat 12-0112-82), FITC-conjugated anti-F4/80 (Cat 11-4801-82), and allophycocyanin-conjugated
anti-CD45 (Cat 17-0451-82). The cells were examined by flow cytometry using a NovoCyte flow cytometer (ACEA Biosciences Inc., San Diego, CA, USA).
