SDS-PAGE Protein Separation

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) was carried out on a 12% polyacrylamide resolving gel with a 4% acrylamide stacking gel. After loading with 20 μg of sample per lane, and a Prestained SDS-PAGE Standard, broad range (Cat. #161-0318; Bio-Rad, Hercules, CA, USA), the gels were run in a Mini-PROTEAN Tetra Cell (Bio-Rad, USA) at 140 V. Gels were stained for 60 min (Coomassie Brilliant Blue R-250; Bio-Rad, USA) and destained overnight.

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Kim H, & Chin K.B. (2024). Effects of gold and green kiwifruit juices on the physicochemical properties and tenderness of pork loin and antioxidant activity during incubation (24 h) in a pork model system. Animal Bioscience, 37(5), 908-917.

Publication 2024

Mini-PROTEAN Tetra Cell Coomassie Brilliant Blue R-250

Corresponding Organization : Chonnam National University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein band patterns on the SDS-PAGE gel

control variables

Polyacrylamide gel composition (12% resolving gel, 4% stacking gel)
Prestained SDS-PAGE Standard, broad range (Cat. #161-0318; Bio-Rad, Hercules, CA, USA) as a molecular weight marker
Voltage applied during electrophoresis (140 V)
Staining and destaining procedures (Coomassie Brilliant Blue R-250 staining for 60 min, destaining overnight)

controls

Prestained SDS-PAGE Standard, broad range (Cat. #161-0318; Bio-Rad, Hercules, CA, USA) as a positive control for protein molecular weight determination

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