Arhgef11 Overexpression Rescue Assay

All mice were maintained in a barrier facility with 12-hour light/dark cycles. Mice were genotyped by PCR and both males and females were analyzed. TRE-Arhgef11^CA were generated in the lab as previously described [24 (link)]. Other animal strains used in this study were Villin-rtTA (Jackson Laboratories, [42 (link)]), VillinCre (Jackson Laboratories [43 (link)]), and Rosa-rtTA3 (Jackson Laboratories). For drug rescue experiments, mice were injected with Y-27632 dihydrochloride (ApexBio A3008-50) (10 mg/kg), at 8 hours after doxycycline chow.

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Hinnant T., Ning W, & Lechler T. (2024). Compartment specific responses to contractility in the small intestinal epithelium. PLOS Genetics, 20(3), e1010899.

Publication 2024

Villin-rtTA VillinCre Rosa-rtTA3

Corresponding Organization : Center for Life Sciences

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Variable analysis

independent variables

Genotype: TRE-Arhgef11^CA

dependent variables

Not explicitly mentioned

control variables

Light/dark cycles (12-hour)
Sex (both males and females)

controls

Positive control: Villin-rtTA, VillinCre, and Rosa-rtTA3 mouse strains
Negative control: Not explicitly mentioned

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