The levels of HIV-1 viral RNA were measured and analyzed at designated time points using the automated COBAS Ampliprep system v2.0/Taqman-48 system (Roche Molecular Diagnostics, Basel, Switzerland) as described previously [72 (link)], and plotted using Prism V9.4.1 software. The assay’s detection limit is 200 viral RNA copies/mL, reflective of the known dilution factors [71 (link)].
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