Comprehensive Serum Biomarker Profiling

Serum samples were used to perform the following measurements, through automatic validated methods and equipment (Hitachi 717 analyzer, Roche Diagnostics GMBH, Mannheim, Germany), as previously described [50 (link)]: postprandial glucose, triglycerides (TGs), total-cholesterol (Total-C), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) levels, as well as serum glutamic-oxaloacetic (GOT) and glutamic-pyruvic (GPT) transaminases concentrations. Hemoglobin A1c (HbA1c) levels were determined using the DCA 2000+ analyzer (Bayer Diagnostics, Barcelona, Spain), according to the manufacturer’ instructions. Serum insulin levels were determined by Enzyme-Linked ImmunoSorbent Assay (ELISA) using commercially available kits for rat samples from Mercodia (Uppsala, Sweden). Insulin resistance was evaluated by the homeostatic model assessment of insulin resistance (HOMA-IR) index, which was calculated as previously described [51 (link)], using the following formula: HOMA-IR index = [fasting glucose (mmol/L) × fasting insulin (µU/L)]/22.5. High-sensitivity C-reactive protein (hs-CRP) was assayed by using a rat-specific Elisa kit (MBS764381 from Mybiosource, San Diego, CA, USA) according to the manufacturer’ instructions.

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Nunes S., Viana S.D., Preguiça I., Alves A., Fernandes R., Teodoro J.S., Figueirinha A., Salgueiro L., Silva S., Jarak I., Carvalho R.A., Cavadas C., Rolo A.P., Palmeira C.M., Pintado M.M, & Reis F. (2020). Blueberry Consumption Challenges Hepatic Mitochondrial Bioenergetics and Elicits Transcriptomics Reprogramming in Healthy Wistar Rats. Pharmaceutics, 12(11), 1094.

Publication 2020

DCA 2000+ analyzer

Cholesterol Diagnostics Elisa Glucose Hemoglobin a1c High density lipoprotein cholesterol Homeostatic Hs crp Insulin Insulin resistance Low density lipoprotein cholesterol Serum Transaminases Triglycerides

Corresponding Organization : University of Coimbra

Other organizations : Polytechnic Institute of Coimbra, Rede de Química e Tecnologia, Universidade Católica Portuguesa, Universidade do Porto

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Postprandial glucose
Triglycerides (TGs)
Total-cholesterol (Total-C)
Low-density lipoprotein cholesterol (LDL-C)
High-density lipoprotein cholesterol (HDL-C)
Serum glutamic-oxaloacetic (GOT) and glutamic-pyruvic (GPT) transaminases concentrations
Hemoglobin A1c (HbA1c) levels
Serum insulin levels
Insulin resistance (HOMA-IR index)
High-sensitivity C-reactive protein (hs-CRP)

control variables

Not explicitly mentioned

controls

Not explicitly mentioned

Annotations

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