Retinal Layer Thickness Imaging Protocol

Optical coherence tomography imaging was performed as described before (Sergeys et al., 2019 (link); Vandenabeele et al., 2021 (link)). Briefly, after pupil dilatation with tropicamide (0.5%, Tropicol, Théa), the retina of anesthetized animals was imaged (1000 A-scans, 100 B-scans, 1.4 × 1.4 mm, Bioptigen Envisu R2200). Retinal layer thickness was measured using InVivoVue Diver (v 3.0.8, Bioptigen) software, at 16 locations in the central retina spaced around the optic nerve head, and averaged per mouse.

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Veys L., Devroye J., Lefevere E., Cools L., Vandenabeele M, & De Groef L. (2021). Characterizing the Retinal Phenotype of the Thy1-h[A30P]α-syn Mouse Model of Parkinson’s Disease. Frontiers in Neuroscience, 15, 726476.

Publication 2021

Tropicol Envisu R2200 InVivoVue Diver

Animals Mouse Optic nerve head Optical coherence tomography Pupil dilatation R2200 Retina Scans Tropicamide

Corresponding Organization : VIB-KU Leuven Center for Brain & Disease Research

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Retinal layer thickness

control variables

Pupil dilatation with tropicamide (0.5%, Tropicol, Théa)
Anesthetized animals
Imaging parameters (1000 A-scans, 100 B-scans, 1.4 × 1.4 mm, Bioptigen Envisu R2200)
Measurement locations (16 locations in the central retina spaced around the optic nerve head)

controls

None explicitly mentioned

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