The procedure was essentially carried out as indicated in earlier work (16 (link)). In brief, following trypsin digestion, peptides were further cleaned using a C18 column and then injected into the UltiMate 3000 RSLC nano HPLC (Thermo Fisher Scientific). This HPLC system is linked to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific) using a Proxeon Nanospray source (Thermo Fisher Scientific).
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