SARS-CoV-2 N Protein Immunostaining

Immunostaining was performed to visualize SARS-CoV-2 N protein in hamster lung tissues as previously described [31 (link)]. In-house rabbit antiserum against SARS-CoV-2 N protein and goat anti-rabbit Alexa Fluor (Thermo Fisher Scientific, Waltham, MA, USA) were used as primary and secondary antibodies, respectively. The nuclei of cell were stained by 4′,6-diamidino-2-phenylindole (DAPI) nucleic acid stain from Thermo Fisher Scientific. Images were acquired with a Carl Zeiss (Dublin, CA, USA) LSM880 system.

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Ye Z.W., Yuan S., Chan J.F., Zhang A.J., Yu C.Y., Ong C.P., Yang D., Chan C.C., Tang K., Cao J., Poon V.K., Chan C.C., Cai J.P., Chu H., Yuen K.Y, & Jin D.Y. (2021). Beneficial effect of combinational methylprednisolone and remdesivir in hamster model of SARS-CoV-2 infection. Emerging Microbes & Infections, 10(1), 291-304.

Publication 2021

4′,6-diamidino-2-phenylindole (DAPI) nucleic acid stain LSM880 system

Antibodies Antiserum Goat Hamster House rabbit Lung Nuclei of cell Nucleic acid Rabbit Sars cov 2 n protein Stain Tissues

Corresponding Organization : University of Hong Kong

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Variable analysis

independent variables

SARS-CoV-2 N protein

dependent variables

Visualization of SARS-CoV-2 N protein in hamster lung tissues

control variables

Hamster lung tissues
In-house rabbit antiserum against SARS-CoV-2 N protein
Goat anti-rabbit Alexa Fluor (Thermo Fisher Scientific, Waltham, MA, USA)
4′,6-diamidino-2-phenylindole (DAPI) nucleic acid stain from Thermo Fisher Scientific
Carl Zeiss (Dublin, CA, USA) LSM880 system

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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