Wild-type zebrafish (Danio rerio; AB type) were raised under standard library conditions, and embryo stages were determined as previously described
[38 (link), 39 (link)]. RPS24 Morpholino (5- TGACAGTGACTGTGTCGTTCATCTT-3), RPS24 control MO (5-TGAAAGTAACTGTGACGGTCGTATT-3), miR-142-3p Morpholino (5-TCCATAAAGTAGGAAACACTACACT-3), miR-142-3p control Mo (5-TCaAaAAAtTAcGAAACgCTACACT-3) and p53 Morpholino (5- GCGCCATTGCTTTGCAAGAATTG-3) were obtained from Gene-Tools, LLC (Philomath, OR, USA). Based on our initial trials, 0.5 ng of RPS24 MO and control MO, 20 umol/L miR-142-3p duplexes (Ribo Company, Guangzhou, China), 2 ng miR-142-3p MO and control MO in Danieau’s buffer were chosen as the optimal concentration, which showed significant decrease in O-staining signal, but no obvious morphological defects when compared with the control embryos. The O-dianisidine (Sigma-Aldrich Company, Saint Louis, USA) staining protocols were as previously described
[18 (link), 40 (link)]. All the studies of zebrafish were approved by the Animal Care and Use Committee of Huazhong University of Science and Technology.
[38 (link), 39 (link)]. RPS24 Morpholino (5- TGACAGTGACTGTGTCGTTCATCTT-3), RPS24 control MO (5-TGAAAGTAACTGTGACGGTCGTATT-3), miR-142-3p Morpholino (5-TCCATAAAGTAGGAAACACTACACT-3), miR-142-3p control Mo (5-TCaAaAAAtTAcGAAACgCTACACT-3) and p53 Morpholino (5- GCGCCATTGCTTTGCAAGAATTG-3) were obtained from Gene-Tools, LLC (Philomath, OR, USA). Based on our initial trials, 0.5 ng of RPS24 MO and control MO, 20 umol/L miR-142-3p duplexes (Ribo Company, Guangzhou, China), 2 ng miR-142-3p MO and control MO in Danieau’s buffer were chosen as the optimal concentration, which showed significant decrease in O-staining signal, but no obvious morphological defects when compared with the control embryos. The O-dianisidine (Sigma-Aldrich Company, Saint Louis, USA) staining protocols were as previously described
[18 (link), 40 (link)]. All the studies of zebrafish were approved by the Animal Care and Use Committee of Huazhong University of Science and Technology.
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