MeCP2 Knockdown in HUVECs

MeCP2 siRNA transfection was performed as previously publication54 (link). Briefly, HUVECs was transfected with 10 nM MeCP2 siRNA(2 hrs) (sc-156056) or scrambled siRNA (sc-37007) (Santa Cruz Biotechnology, Dallas, TX, USA) using HiPerFect Transfection Reagent (QIAGEN) as instructed by the manufacture. 48 hrs after the transfection, the cells were harvested and protein was extracted from the cells for MeCP2 and VEGF expression by western blot analysis.

Free full text: Click here

Li X., Liu X., Guo H., Zhao Z., Li Y.S, & Chen G. (2016). The significance of the increased expression of phosphorylated MeCP2 in the membranes from patients with proliferative diabetic retinopathy. Scientific Reports, 6, 32850.

Publication 2016

scrambled siRNA (sc-37007 HiPerFect Transfection Reagent

Cells Mecp2 Protein Sirna Transfection Vegf Western blot analysis

Corresponding Organization :

Other organizations : Henan Provincial Eye Hospital

Top 5 similar protocols

Variable analysis

independent variables

MeCP2 siRNA transfection

dependent variables

MeCP2 expression
VEGF expression

control variables

Scrambled siRNA transfection

controls

Positive control: Scrambled siRNA transfection
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!