Quantitative RT-PCR Analysis of Inflammatory Genes

Quantitative RT-PCR was performed on samples as previously described [26] (link). Primers were purchased from Applied Biosystems (Carlsbad, CA); CARD9 (Hs00364485_m1), CARD10 (Hs00367225_m1), CARD11 (Hs01060620_m1), CARD14 (Hs00364499_m1), IL-8 (Hs99999034_m1), CCL2 (Hs00234140_m1), CCL5 (Hs00174575_m1), CXCL1 (Hs00236937_m1), CXCL10 (IP10) (Hs99999049_m1), ICAM1 (Hs00164932_m1), VCAM (Hs01003372_m1), E-selectin (Hs00950401_m1), P-selectin (Hs008927900). Gene expression was normalized to the house-keeping gene, human acidic ribosomal protein (hARP).

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Harden J.L., Lewis S.M., Pierson K.C., Suárez-Fariñas M., Lentini T., Ortenzio F.S., Zaba L.C., Goldbach-Mansky R., Bowcock A.M, & Lowes M.A. (2014). CARD14 Expression in Dermal Endothelial Cells in Psoriasis. PLoS ONE, 9(11), e111255.

Publication 2014

CXCL1 ICAM1 E-selectin P-selectin

Acidic Card14 Card9 Ccl2 Ccl5 Cxcl1 E selectin Gene expression House keeping gene Human Human protein Icam1 Ip10 P selectin Primers Ribosomal Ribosomal protein Rt pcr Vcam

Corresponding Organization : Imperial College London

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression of the following genes: CARD9, CARD10, CARD11, CARD14, IL-8, CCL2, CCL5, CXCL1, CXCL10 (IP10), ICAM1, VCAM, E-selectin, P-selectin

control variables

The house-keeping gene, human acidic ribosomal protein (hARP), was used to normalize gene expression.

controls

No positive or negative controls were explicitly mentioned.

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