Murine MC903- and vehicle EtOH-treated skin RNA-seq data were obtained from a previously published study (45 (link)). For sequencing of human skin, 4-mm punch biopsies were placed in RNAlater (Life Technologies) overnight at 4°C and stored at −80°C until further processing. Skin was homogenized with a bead homogenizer in RNA lysis buffer, and RNA was isolated with the RNeasy Mini Kit (Qiagen). Library preparation, alignment, and transcript abundance were performed by the Genome Technology Access Center (GTAC) at WUSM as previously described (45 (link)). Briefly, after deoxyribonuclease treatment (TURBO DNase, Invitrogen), ribosomal RNA was removed with Ribo-Zero kit (MRZH11124; Illumina) and RNA was reverse-transcribed using SuperScript II RT enzyme (Invitrogen). Human samples were sequenced with an average of 60 million 1 × 50 single reads on an Illumina HiSeq3000. Reads were aligned to Ensembl release 76 human genome assembly using STAR (71 (link)), gene counts were determined with Subread:featureCount (72 (link)), and sequence performance was assessed with RSeQC (73 (link)).