Immunochemical Detection of Inflammatory Markers

As described previously [9 (link)], recombinant human DAF (rhDAF) and biotinylated anti-human DAF were obtained from R&D systems (Minneapolis, MN). Antibodies such as mouse anti-HMGB1, mouse anti-rat endothelial cell, rabbit anti-RAGE, Chicken anti-mouse C3/3a, and rabbit anti-NF-κB were purchased from Abcam (Cambridge, MA)., and rabbit anti-caspase-1 antibody was obtained from Cell Signaling Technologies (Danvers, MA). Mouse anti-ratC3a receptor (C3aR) was acquired from Hycult Biotech Inc (Plymouth Meeting, PA). Conjugated secondary antibodies (goat anti-mouse Alexa Fluor 488, goat anti-rabbit 594, goat anti-chicken 594), and ProLong Gold Antifade reagent were obtained from Invitrogen (Carlsbad, CA).

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Li Y., Yang Z., Chavko M., Liu B., Aderemi O.A., Simovic M.O., Dubick M.A, & Cancio L.C. (2018). Complement inhibition ameliorates blast-induced acute lung injury in rats: Potential role of complement in intracellular HMGB1-mediated inflammation. PLoS ONE, 13(8), e0202594.

Publication 2018

rabbit anti-RAGE rabbit anti-NF-κB rabbit anti-caspase-1 antibody goat anti-mouse Alexa Fluor 488 goat anti-chicken 594 ProLong Gold Antifade reagent

Alexa fluor 488 Anti antibodies Anti antibody Antibodies C3ar Caspase 1 Chicken Endothelial cell Goat Gold Hmgb1 Human Mouse Nf κb Rabbit Rage

Corresponding Organization : Naval Medical Research Command

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