Analysis of Spleen and Lymph Node DCs

Spleen and sLN DCs were analyzed as described elsewhere [23 (link),45 (link),46 (link)]. After density cut by 1.077 histopaque from digested spleen as shown in NK cell analysis, the light density fraction (<1.077 g/cm³) was collected and incubated for 30 min with the following FITC-conjugated monoclonal antibodies (mAbs): anti-CD3 (17A2), anti-Thy1.1 (OX-7), anti-B220 (RA3-6B2), anti-Gr1 (RB68C5), anti-CD49b (DX5), and anti-TER-119 (TER-119) (BioLegend, San Diego, CA, USA). The lineage⁻CD11c⁺ cells were defined as DCs. Analysis was carried out on a FACS Aria II (Becton Dickinson, San Diego, CA, USA).

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Wang Y., Zhang W., Xu L, & Jin J.O. (2016). Porphyromonas gingivalis Lipopolysaccharide Induced Proliferation and Activation of Natural Killer Cells in Vivo. Molecules, 21(8), 1086.

Publication 2016

anti-TER-119 (TER-119) FACS Aria II

Anti cd3 Anti thy1 Aria Cells Fitc Histopaque Light Monoclonal antibodies Nk cell Spleen

Corresponding Organization : Shanghai Public Health Clinical Center

Other organizations : Shanghai Jiao Tong University, Shanghai Ninth People's Hospital

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Variable analysis

independent variables

Density cut by 1.077 histopaque from digested spleen

dependent variables

Spleen and sLN DCs

control variables

Incubation time of 30 min with FITC-conjugated monoclonal antibodies
FACS Aria II (Becton Dickinson, San Diego, CA, USA) for analysis

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

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