To evaluate the effect of C-MSC-Exo on cardiac repair after induction of
myocardial infarction, mice were subjected to acute MI as previously
described(38 (link), 39 (link)). Briefly, C57BL/6 mice were anesthetized with an
intraperitoneal injection of 100 mg/kg ketamine and 10 mg/kg xylazine. The mice
were orally intubated with a 24 gauge tube and ventilated with room air using a
Harvard Rodent Ventilator (Model 55–7058, Holliston, MA). The thorax was
opened by a lateral thoracotomy and the heart was exposed by a pericardial
incision. An 8–0 nylon suture (Ethicon, Somerville, NJ) was placed under
the left anterior inferior artery (LAD) for permanent ligation. Immediately
after coronary occlusion, mice were injected intramuscularly with 30 μl
PBS or C-MSC-Exo (50 μg, 30 μl) in the infarct border zone. The
chest was closed by layers, and the mice were allowed to recover. Animals were
sacrificed one month after LAD ligation for tissue harvesting and histological
assay. Animals were treated according to approved protocols and animal welfare
regulations of the Institutional Animal Care and Use Committee of the Medical
College of Georgia.
myocardial infarction, mice were subjected to acute MI as previously
described(38 (link), 39 (link)). Briefly, C57BL/6 mice were anesthetized with an
intraperitoneal injection of 100 mg/kg ketamine and 10 mg/kg xylazine. The mice
were orally intubated with a 24 gauge tube and ventilated with room air using a
Harvard Rodent Ventilator (Model 55–7058, Holliston, MA). The thorax was
opened by a lateral thoracotomy and the heart was exposed by a pericardial
incision. An 8–0 nylon suture (Ethicon, Somerville, NJ) was placed under
the left anterior inferior artery (LAD) for permanent ligation. Immediately
after coronary occlusion, mice were injected intramuscularly with 30 μl
PBS or C-MSC-Exo (50 μg, 30 μl) in the infarct border zone. The
chest was closed by layers, and the mice were allowed to recover. Animals were
sacrificed one month after LAD ligation for tissue harvesting and histological
assay. Animals were treated according to approved protocols and animal welfare
regulations of the Institutional Animal Care and Use Committee of the Medical
College of Georgia.
