Immunocytochemistry and Immunohistochemistry of MPC1/MPC2

Cytoblocks were prepared for ICC and DakoEnVision™ Flex+ (K8012; Dako, Glostrup, Denmark) was applied for ICC and IHC staining as previously described [43 (link)]. After deparaffinization, unmasking epitopes and blocking of peroxidase, the slides were then incubated with the following reagents: rabbit monoclonal antibody against human MPC1(HPA045119, Sigma-Aldrich, St. Louis, MO, USA, diluted 1:500) and MPC2(ab111380, abcam, Cambridge, UK, diluted 1:500) at 4°C overnight. Negative controls were produced using the same concentration of non-immune rabbit IgG instead of the rabbit antibody to human MPC1 and MPC2.

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Li Y., Li X., Kan Q., Zhang M., Li X., Xu R., Wang J., Yu D., Goscinski M.A., Wen J.G., Nesland J.M, & Suo Z. (2016). Mitochondrial pyruvate carrier function is negatively linked to Warburg phenotype in vitro and malignant features in esophageal squamous cell carcinomas. Oncotarget, 8(1), 1058-1073.

Publication 2016

DakoEnVision™ Flex+ HPA045119 ab111380

Antibody Epitopes Human Monoclonal antibody Peroxidase Rabbit

Corresponding Organization : University of Oslo

Other organizations : Anyang Tumor Hospital

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Variable analysis

independent variables

Rabbit monoclonal antibody against human MPC1 (diluted 1:500)
Rabbit monoclonal antibody against human MPC2 (diluted 1:500)

dependent variables

ICC and IHC staining results

control variables

Deparaffinization
Unmasking epitopes
Blocking of peroxidase

controls

Negative controls: Non-immune rabbit IgG instead of the rabbit antibody to human MPC1 and MPC2

Annotations

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