Extraction and Yield of Phytochemicals

The collected sampls were sliced into smaller pieces and dried at room temperature under shade and then powdered finely and subjected to extraction. Five hundred grams of the powder was macerated with about 2500 ml of 80% methanol for 15 days. The extract was then filtered and the marc was remacerated twice using the same volume of solvent to exhaustively extract the plant material [19 (link)]. The methanol was then removed from the extract by evaporation under reduced pressure using a rota vapor (BUCHI Rotavapour R-200, Switzerland) at 40 °C. The resulting dry hydroalcoholic extract was weighed and calculated for percentage yield, which was 23% (w/w). The dried plant extract was reconstituted with distilled water for oral administration.