Frozen Kidney cAMP Quantification

The frozen kidneys were ground to a fine powder with liquid nitrogen in a stainless-steel mortar. After the liquid nitrogen had evaporated, the tissues were assayed for cAMP using an enzyme-linked immunosorbent assay kit (Enzo Life Sciences Inc., Farmingdale, NY) (29 (link)). Results are expressed in picomole per milligram of tissue protein.

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QIU J., SATO Y., XU L., MIURA T., KOHZUKI M, & ITO O. (2021). Chronic Exercise Protects against the Progression of Renal Cyst Growth and Dysfunction in Rats with Polycystic Kidney Disease. Medicine and Science in Sports and Exercise, 53(12), 2485-2494.

Publication 2021

enzyme-linked immunosorbent assay kit

Enzyme linked immunosorbent assay Frozen Kidneys Nitrogen Powder Protein Stainless steel Tissue

Corresponding Organization :

Other organizations : Tohoku University, Tohoku Medical and Pharmaceutical University

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Variable analysis

independent variables

Grinding of the frozen kidneys to a fine powder with liquid nitrogen in a stainless-steel mortar

dependent variables

CAMP (cyclic adenosine monophosphate) levels in the tissue samples

control variables

Evaporation of the liquid nitrogen after grinding the tissue samples

controls

None explicitly mentioned

Annotations

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