The retina from formalin-fixed eyes of ZDF and lean rats was rinsed overnight with running water, digested with 3% crude trypsin (Invitrogen-Gibco, Grand Island, NY, USA) containing 200 mM sodium fluoride. Trypsin-digested microvasculature was mounted on a glass slide, and stained with periodic acid Schiff-hematoxylin to count the degenerative capillaries.18 (link)
Protocol detail
Quantifying Diabetic Retinal Capillary Degeneration
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Capillaries
Fixed formalin
Formalin
Microvasculature
Periodic acid
Rats
Retina
Sodium fluoride
Trypsin
Corresponding Organization : Wayne State University
Other organizations : Michigan United
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Variable analysis
independent variables
- Type of rat (ZDF vs. lean)
- Number of degenerative capillaries
- Formalin-fixed eyes
- Overnight rinsing with running water
- 3% crude trypsin digestion
- 200 mM sodium fluoride in trypsin
- Periodic acid Schiff-hematoxylin staining
- No positive or negative controls were explicitly mentioned in the provided information.
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