Ptk6 null mice (B6-Ptk6tm1Aty) in the C57BL/6 strain19 (link) were backcrossed with the FVB/N inbred strain (Harlan Laboratories, Frederick, MD, USA) for at least eight generations to generate Ptk6 null mice in FVB/N background. FVB-Ptk6tm1Aty were then crossed with FVB-MMTV-ERBB2 transgenic mice48 (link) (Jackson Laboratories, Bar Harbor, ME, USA, Stock number 005038, FVB-Tg(MMTV-Erbb2)NK1Mul/J) to produce ERBB2;Ptk6−/− animals. These mice carry activated rat c-Neu (Erbb2) (Val664 to Glu664) under the control of the MMTV long-terminal repeat (LTR).
Tissues from age-matched female mice were used in all experiments, and FVB/NJ nontransgenic mice were used for maintaining the lines and nontransgenic controls. Animals were palpated one or two times a week for subcutaneous mass from the age of 8 weeks.
Tissues from age-matched female mice were used in all experiments, and FVB/NJ nontransgenic mice were used for maintaining the lines and nontransgenic controls. Animals were palpated one or two times a week for subcutaneous mass from the age of 8 weeks.
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