Eight ml of ventricular CSF was collected during the surgical intervention, immediately after shunt insertion and a discard of the first 2 ml. All CSF analyses were performed at the Neurochemistry Laboratory at Sahlgrenska University Hospital, by board-certified laboratory technicians who were blinded to clinical data. Aβ-related biomarkers (Aβ40, Aβ42, sAPPα and sAPPβ) and MCP1 were analyzed by electrochemiluminescence assays (Meso Scale Discovery, Rockville, MD, USA). Validated in-house ELISA methodology was used to analyze NfL [19 (link)), neurogranin [15 (link)), GAP43 [20 (link)) and GFAP [21 (link)), whereas CSF levels of T-tau, and P-tau were measured using commercially available Lumipulse technology (Fujirebio, Ghent, Belgium), as previously described [22 (link)]. YKL-40 was measured using Human Chitinase 3-like 1 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN) [22 (link)]. All concentrations are given in ng/L. All samples were analyzed in one round of experiments using one batch of reagents by board-certified laboratory technicians who were blinded to clinical data. Intra-assay coefficients of variation, monitored using internal quality control samples in the beginning and end of each run, were below 10%.
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